استخراج آنتی ژن های دفعی- ترشحی انگل توکسوپلاسما گوندی در طراحی کیت الایزا اویدیتی (Elisa avidity)

Background and purpose: One of the transmission ways of the congenital toxoplasmosis is through the placenta to the fetus, therefore, its diagnosis at early stage is very important. Toxoplasma gondii parasite contains various antigens of which excretory-secretory antigens (E/SA) are used in designing Elisa avidity kit. The aim of this study was to compare the results of commercial kit and designed Elisa avidity kit in mothers with toxoplasmosis. Excretory-secretory antigens of toxoplasma gondii parasite were used in the designed kit. Materials and methods: In this descriptive study aspirated peritoneal fluid of mice which were IP infected by toxoplasma tachyzoite (106 tachyzoites/mL) seven days earlier, were centrifuged at 1000g for 15 minutes. Afterwards, the upper fluid was taken as the main component of E/SA from toxoplasma tachyzoite in order to assess the designed Elisa avidity kit. Results: The results showed that if fresh E/SA are used in designed Elisa avidity kit to detect toxoplasma IgG, better outcomes may found regarding the quantity and titer of antibody. Conclusion: The results drawn from the designed kit using E/SA of toxoplasma gondii were more reliable than that of the commercial kit in detecting congenital toxoplasmosis.